Recirculating fluid saves culture medium, which can be highly expensive for primary cells, and enriches the medium with cell-secreted factors. Most peristaltic pumps are designed to fit within an incubator, and the latest generation of micropumps are highly compact (e.g., MP² micropumps from Elemental Scientific, Omaha, NE, United States, and RP-TX microperistaltic pumps from Takasago Fluidic Systems, Kobe, Japan). In vitro patient-derived 3D mesothelioma tumor organoids facilitate patient-centric therapeutic screening. Reproduced under a Creative Commons Attributions 4.0 International License from Mazzocchi, A.R., Rajan, S.A.P., Votanopoulos, K.I., Hall, A.R., Skardal, A., 2018. Scheme of a six-chamber cell-based assay with a low-volume closed-loop fluidic circuit perfusing each sample. Tube-pinching by the rollers damages the tubes, which must be replaced periodically, and this type of pump should not be used in experiments involving circulating cells.įigure 3.10. In most cases, all tubes are coiled around one rotor and therefore deliver the same flow rate in every chip, which can be limiting for certain studies. Most complex flow patterns, including sinusoidal flow, are difficult to achieve because of flow pulsation. Most peristaltic pumps are software-driven to automate protocols such as periodic injection or simple flow variations, the latter including median flow rate increase or decrease ( Mazzocchi et al., 2018). The frequency and amplitude of oscillations required to obtain a given flow rate vary between pumps by the number of rollers, their rotation frequency, and the diameter of the tubes. Peristaltic pumps deliver pulsatile flow. The number of samples that can be perfused in parallel varies from 1 to 24, depending on the number of tubes that can be coiled around the pump rotor. They can either be used to pump medium from source to waste or to recirculate medium in a chip. Peristaltic pumps are widely used in cell culture as they are compact and easy to use and connect ( Caplin et al., 2015 Maschmeyer et al., 2015a Kieninger et al., 2018 Alexander et al., 2018a Moya et al., 2018) ( Fig.
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